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antimouse cd11c fitc  (Miltenyi Biotec)
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Fig. 5. Immunohistochemistry of the intestinal epithelium from DSS-induced murine colitis. (A) Assessment of immune cell infiltrates in distal colon cross sections from uninduced control mice (Blank) and DSS-induced mice either untreated (DSS), C4BP(β-)-treated (C4BP(β-)), or minocycline-treated (Minocycline) at the end of the study. Hyperplasic villi and immune cell infiltration of crypts were apparent in DSS-induced, untreated mice surveyed for dendritic cells <t>(CD11c),</t> monocytes/ macrophages (F4/80), and activated B cells (BAFFR). Particularly, colonic tissue from C4BP(β-)-treated mice was found scarcely stained for the above-referred inflammatory markers. Bars = 50 µm. (B) Staining was quantitatively assessed by digital image analysis through Image J and the IHC Profiler plugin. The results shown are the mean ± SD of 3,3’-diaminobenzidine positive pixels (%) stained for each of the tested markers on the colonic sections (n = 6 mice/group) (*, p < 0.05, **, p < 0.01; ***, p < 0.001 compared with DSS colitic mice).
Rabbit Polyclonal Antimouse Cd11c Antibody, supplied by Biorbyt, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 5. Immunohistochemistry of the intestinal epithelium from DSS-induced murine colitis. (A) Assessment of immune cell infiltrates in distal colon cross sections from uninduced control mice (Blank) and DSS-induced mice either untreated (DSS), C4BP(β-)-treated (C4BP(β-)), or minocycline-treated (Minocycline) at the end of the study. Hyperplasic villi and immune cell infiltration of crypts were apparent in DSS-induced, untreated mice surveyed for dendritic cells <t>(CD11c),</t> monocytes/ macrophages (F4/80), and activated B cells (BAFFR). Particularly, colonic tissue from C4BP(β-)-treated mice was found scarcely stained for the above-referred inflammatory markers. Bars = 50 µm. (B) Staining was quantitatively assessed by digital image analysis through Image J and the IHC Profiler plugin. The results shown are the mean ± SD of 3,3’-diaminobenzidine positive pixels (%) stained for each of the tested markers on the colonic sections (n = 6 mice/group) (*, p < 0.05, **, p < 0.01; ***, p < 0.001 compared with DSS colitic mice).
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Fig. 5. Immunohistochemistry of the intestinal epithelium from DSS-induced murine colitis. (A) Assessment of immune cell infiltrates in distal colon cross sections from uninduced control mice (Blank) and DSS-induced mice either untreated (DSS), C4BP(β-)-treated (C4BP(β-)), or minocycline-treated (Minocycline) at the end of the study. Hyperplasic villi and immune cell infiltration of crypts were apparent in DSS-induced, untreated mice surveyed for dendritic cells <t>(CD11c),</t> monocytes/ macrophages (F4/80), and activated B cells (BAFFR). Particularly, colonic tissue from C4BP(β-)-treated mice was found scarcely stained for the above-referred inflammatory markers. Bars = 50 µm. (B) Staining was quantitatively assessed by digital image analysis through Image J and the IHC Profiler plugin. The results shown are the mean ± SD of 3,3’-diaminobenzidine positive pixels (%) stained for each of the tested markers on the colonic sections (n = 6 mice/group) (*, p < 0.05, **, p < 0.01; ***, p < 0.001 compared with DSS colitic mice).
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apc antimouse cd11c antibody  (Proteintech)
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Fig. 5. Immunohistochemistry of the intestinal epithelium from DSS-induced murine colitis. (A) Assessment of immune cell infiltrates in distal colon cross sections from uninduced control mice (Blank) and DSS-induced mice either untreated (DSS), C4BP(β-)-treated (C4BP(β-)), or minocycline-treated (Minocycline) at the end of the study. Hyperplasic villi and immune cell infiltration of crypts were apparent in DSS-induced, untreated mice surveyed for dendritic cells <t>(CD11c),</t> monocytes/ macrophages (F4/80), and activated B cells (BAFFR). Particularly, colonic tissue from C4BP(β-)-treated mice was found scarcely stained for the above-referred inflammatory markers. Bars = 50 µm. (B) Staining was quantitatively assessed by digital image analysis through Image J and the IHC Profiler plugin. The results shown are the mean ± SD of 3,3’-diaminobenzidine positive pixels (%) stained for each of the tested markers on the colonic sections (n = 6 mice/group) (*, p < 0.05, **, p < 0.01; ***, p < 0.001 compared with DSS colitic mice).
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antimouse cd11c antibody  (Becton Dickinson)
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Fig. 5. Immunohistochemistry of the intestinal epithelium from DSS-induced murine colitis. (A) Assessment of immune cell infiltrates in distal colon cross sections from uninduced control mice (Blank) and DSS-induced mice either untreated (DSS), C4BP(β-)-treated (C4BP(β-)), or minocycline-treated (Minocycline) at the end of the study. Hyperplasic villi and immune cell infiltration of crypts were apparent in DSS-induced, untreated mice surveyed for dendritic cells <t>(CD11c),</t> monocytes/ macrophages (F4/80), and activated B cells (BAFFR). Particularly, colonic tissue from C4BP(β-)-treated mice was found scarcely stained for the above-referred inflammatory markers. Bars = 50 µm. (B) Staining was quantitatively assessed by digital image analysis through Image J and the IHC Profiler plugin. The results shown are the mean ± SD of 3,3’-diaminobenzidine positive pixels (%) stained for each of the tested markers on the colonic sections (n = 6 mice/group) (*, p < 0.05, **, p < 0.01; ***, p < 0.001 compared with DSS colitic mice).
Antimouse Cd11c Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antimouse cd11c+ antibody clone: n418  (Thermo Fisher)
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Thermo Fisher antimouse cd11c+ antibody clone: n418
Fig. 5. Immunohistochemistry of the intestinal epithelium from DSS-induced murine colitis. (A) Assessment of immune cell infiltrates in distal colon cross sections from uninduced control mice (Blank) and DSS-induced mice either untreated (DSS), C4BP(β-)-treated (C4BP(β-)), or minocycline-treated (Minocycline) at the end of the study. Hyperplasic villi and immune cell infiltration of crypts were apparent in DSS-induced, untreated mice surveyed for dendritic cells <t>(CD11c),</t> monocytes/ macrophages (F4/80), and activated B cells (BAFFR). Particularly, colonic tissue from C4BP(β-)-treated mice was found scarcely stained for the above-referred inflammatory markers. Bars = 50 µm. (B) Staining was quantitatively assessed by digital image analysis through Image J and the IHC Profiler plugin. The results shown are the mean ± SD of 3,3’-diaminobenzidine positive pixels (%) stained for each of the tested markers on the colonic sections (n = 6 mice/group) (*, p < 0.05, **, p < 0.01; ***, p < 0.001 compared with DSS colitic mice).
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Image Search Results


Fig. 5. Immunohistochemistry of the intestinal epithelium from DSS-induced murine colitis. (A) Assessment of immune cell infiltrates in distal colon cross sections from uninduced control mice (Blank) and DSS-induced mice either untreated (DSS), C4BP(β-)-treated (C4BP(β-)), or minocycline-treated (Minocycline) at the end of the study. Hyperplasic villi and immune cell infiltration of crypts were apparent in DSS-induced, untreated mice surveyed for dendritic cells (CD11c), monocytes/ macrophages (F4/80), and activated B cells (BAFFR). Particularly, colonic tissue from C4BP(β-)-treated mice was found scarcely stained for the above-referred inflammatory markers. Bars = 50 µm. (B) Staining was quantitatively assessed by digital image analysis through Image J and the IHC Profiler plugin. The results shown are the mean ± SD of 3,3’-diaminobenzidine positive pixels (%) stained for each of the tested markers on the colonic sections (n = 6 mice/group) (*, p < 0.05, **, p < 0.01; ***, p < 0.001 compared with DSS colitic mice).

Journal: Pharmacological research

Article Title: C4BP(β-)-mediated immunomodulation attenuates inflammation in DSS-induced murine colitis and in myeloid cells from IBD patients.

doi: 10.1016/j.phrs.2023.106948

Figure Lengend Snippet: Fig. 5. Immunohistochemistry of the intestinal epithelium from DSS-induced murine colitis. (A) Assessment of immune cell infiltrates in distal colon cross sections from uninduced control mice (Blank) and DSS-induced mice either untreated (DSS), C4BP(β-)-treated (C4BP(β-)), or minocycline-treated (Minocycline) at the end of the study. Hyperplasic villi and immune cell infiltration of crypts were apparent in DSS-induced, untreated mice surveyed for dendritic cells (CD11c), monocytes/ macrophages (F4/80), and activated B cells (BAFFR). Particularly, colonic tissue from C4BP(β-)-treated mice was found scarcely stained for the above-referred inflammatory markers. Bars = 50 µm. (B) Staining was quantitatively assessed by digital image analysis through Image J and the IHC Profiler plugin. The results shown are the mean ± SD of 3,3’-diaminobenzidine positive pixels (%) stained for each of the tested markers on the colonic sections (n = 6 mice/group) (*, p < 0.05, **, p < 0.01; ***, p < 0.001 compared with DSS colitic mice).

Article Snippet: For the immunohistochemical analysis, sample slides were incubated overnight at 4 oC with the primary antibodies: rabbit polyclonal antimouse CD11c antibody (1:200) (Biorbyt, Cambridge, UK), rabbit polyclonal anti-mouse BAFFR antibody (1:200) (ThermoFisher), rat monoclonal antibody anti-mouse F4/80 (1:50) (ThermoFisher).

Techniques: Immunohistochemistry, Control, Staining